In the common order of draw, green heparin tubes are collected after serum tubes and before lavender or pink EDTA tubes. Is this statement true or false?
3 Order of Draw Online Quiz Questions
Use this free practice quiz with 20 questions to review 3 Order of Draw, test your knowledge, and prepare for your next test or exam.
What additive is commonly used in light-blue tubes for coagulation tests?
A specimen is requested for a plasma chemistry test. Which common tube type is associated with that use?
- A
A red serum tube
- B
A green heparin tube
- C
A lavender EDTA tube
- D
A gray fluoride/oxalate tube
For coagulation testing, a light-blue citrate tube must be filled to its indicated volume to preserve the .
When a discard tube is used only to clear air from winged-set tubing before a light-blue citrate specimen, the discard tube does not need to be filled. Is this statement true or false?
- A
True
- B
False
A collector is obtaining a blood culture, a light-blue coagulation specimen, a serum specimen, and an EDTA specimen during one venipuncture. Which sequence follows the common order of draw?
- A
Light blue → blood culture → EDTA → serum
- B
Serum → EDTA → light blue → blood culture
- C
Blood culture → light blue → serum → EDTA
- D
EDTA → blood culture → serum → light blue
After filling an additive tube, what gentle mixing action should the collector use as directed by the manufacturer?
Blood-culture bottles are collected first to help protect culture specimens from by material in other collection tubes.
Which actions help maintain specimen integrity during collection? Select all that apply.
- A
Fill tubes to the indicated volume, especially citrate tubes.
- B
Do not transfer blood between collection tubes.
- C
Shake additive tubes vigorously to mix them.
- D
Gently invert additive tubes as directed by the manufacturer.
A collector must place a specialty tube whose cap color alone does not establish its position. What should guide its placement?
- A
Place it based only on its cap color.
- B
Check its additive and follow the laboratory's instructions.
- C
Always place it after the gray tube.
- D
Treat all specialty tubes as serum tubes.
Which statements correctly describe possible effects of additive carryover? Select all that apply.
- A
EDTA contamination can interfere with calcium and potassium measurements.
- B
EDTA contamination is used to preserve accurate calcium measurements.
- C
Heparin or citrate carryover can affect coagulation testing.
- D
Additive carryover cannot change a test result if the tube is filled correctly.
A collector notices that one tube is underfilled and proposes moving blood from another collection tube into it. Which response follows the specimen-handling guidance?
- A
Transfer blood from a full tube to the underfilled tube.
- B
Do not transfer blood between tubes; follow the laboratory's collection instructions.
- C
Combine the specimens and divide them evenly between the tubes.
- D
Shake both tubes and then transfer enough blood to balance them.
Explain why the order of draw matters when several blood tubes are collected during one venipuncture. Include one example of how contamination between tubes could affect a specimen or test result.
Tube color and additive formulation can vary by manufacturer and test, so the laboratory's current collection instructions should take precedence when they differ from the general sequence. Is this statement true or false?
- A
True
- B
False
A specimen is being collected for glucose-related testing. Which additive combination is commonly associated with a gray tube?
- A
Sodium citrate
- B
Sodium fluoride with potassium oxalate
- C
Heparin
- D
EDTA
Why are blood-culture bottles collected before other tubes in the standard order of draw?
- A
To preserve the blood-to-additive ratio in citrate tubes
- B
To prevent EDTA from affecting potassium measurements
- C
To help protect culture specimens from contamination by material in other tubes
- D
To ensure serum tubes clot before collection
A laboratory requests a green tube for a plasma chemistry specimen. What additive is commonly used in this tube?
During one venipuncture, a collector needs blood cultures, a light-blue coagulation tube, a serum tube, and an EDTA tube. Which sequence follows the standard order of draw?
- A
Light blue → blood culture → serum → EDTA
- B
Blood culture → light blue → serum → EDTA
- C
Blood culture → serum → light blue → EDTA
- D
Serum → blood culture → light blue → EDTA
A collector notices that a light-blue citrate tube is underfilled. Why is filling it to the indicated volume especially important?
- A
The blood-to-additive ratio is important for coagulation testing.
- B
Citrate must be kept from contact with serum separator gel.
- C
The tube needs enough air space for gentle inversion.
- D
The additive works only if the tube is filled after an EDTA tube.
A light-blue citrate tube is the first specimen collected with a winged set, and air in the tubing may prevent it from filling adequately. What type of tube may local procedure call for to clear the tubing?