True or false: Serum is obtained after blood has clotted, whereas plasma is collected with an anticoagulant and retains clotting factors.
3 Specimen Processing and Centrifugation Online Quiz Questions
Use this free practice quiz with 20 questions to review 3 Specimen Processing and Centrifugation, test your knowledge, and prepare for your next test or exam.
During inspection, you find a cracked specimen tube. What should you do before centrifugation?
- A
Use it if the crack is small and the tube fits the rotor.
- B
Do not use it; select an undamaged, compatible tube.
- C
Wrap it in absorbent material and use it only at low speed.
- D
Use it only if the centrifuge lid is closed.
Which abbreviation names the centrifugation setting preferred over RPM because it specifies the force applied to the specimen?
To balance a centrifuge with two tubes of equal mass, place them one another.
A blood specimen is collected in a serum tube. What should happen before centrifugation?
- A
Centrifuge it immediately so clotting occurs during the spin.
- B
Add an anticoagulant, then treat it as a serum specimen.
- C
Allow it to clot before centrifugation, following the test instructions.
- D
Shake it vigorously before centrifugation.
Before starting a centrifuge, which actions are appropriate? Select all that apply.
- A
Confirm that tubes, buckets, and rotor components are compatible.
- B
Begin the run with a visibly unbalanced rotor if the run is brief.
- C
Keep the bowl and tubes dry and ensure the rotor is properly seated.
- D
Use safety cups or a sealed rotor when required by the risk assessment and equipment instructions.
- E
Open the centrifuge lid briefly during operation to check the load.
True or false: Using the same RPM in two rotors with different radii will produce the same relative centrifugal force.
- A
True
- B
False
What type of containment should secure the primary specimen container during transport to help contain leaks?
A correctly labeled specimen arrives in the correct container, but there is not enough material for the requested test. What is the appropriate response?
- A
Increase the centrifuge speed to obtain more specimen.
- B
Follow the laboratory's test-specific acceptance and reporting procedures.
- C
Add fluid to the tube until the requested volume is reached.
- D
Transfer the specimen to a different tube and relabel it.
In the RCF relationship, the rotational radius r is measured in .
You have separated a specimen, but the appropriate storage condition is not obvious. Which actions are appropriate? Select all that apply.
- A
Use the temperature and storage period specified for the test.
- B
Refrigerate every separated specimen, regardless of the test.
- C
Choose a storage temperature based on the centrifuge setting.
- D
Contact the responsible laboratory if the instructions are unclear.
A centrifuge begins vibrating and making an unusual noise during a run. What should you do, and when may you investigate the problem?
At specimen collection, which practice helps preserve the link between the specimen and the requested tests?
- A
Label the container after centrifugation to avoid handling it at collection.
- B
Use the tube type to identify the patient if the label is incomplete.
- C
Label the container at collection and verify that the label matches the requisition.
- D
Proceed if the requisition and container have different identifiers, then correct the mismatch later.
True or false: The centrifuge lid should remain closed while the centrifuge is operating.
- A
True
- B
False
A specimen container's label conflicts with the requisition. What is the appropriate action before processing?
- A
Change the container label to match the requisition.
- B
Report the discrepancy and follow the laboratory's identification and acceptance procedures.
- C
Process the specimen, then ask the requester to confirm its identity.
- D
Use the collection time to decide which identification is correct.
A specimen is ready for transport, but its required storage temperature is not known. What should you do?
- A
Refrigerate every specimen during transport.
- B
Keep every specimen at room temperature until testing.
- C
Check the test-specific transport and temperature requirements.
- D
Freeze every specimen if transport will take longer than an hour.
Which statement correctly distinguishes serum from plasma in a blood specimen?
- A
Serum is obtained from anticoagulated blood; plasma is obtained after blood clots.
- B
Serum is obtained after blood clots; plasma is obtained from anticoagulated blood.
- C
Serum and plasma are interchangeable names for the liquid from a blood specimen.
- D
Plasma is obtained only after the red blood cells have clotted.
You have an even number of centrifuge positions to load. Which approach best balances the load?
- A
Place tubes of equal mass opposite one another and load positions symmetrically.
- B
Place the fullest tubes together so their contents settle evenly.
- C
Use any tube as a balance tube, even if its contents are incompatible.
- D
Run the rotor briefly to check whether the load is balanced.
Use RCF=1.118×10−5×r×RPM2 to calculate the RCF for a rotational radius of 8.0 cm and a speed of 3000 RPM. Enter the numerical RCF value in × g.
During specimen transport, what should suitable secondary containment help contain if the primary container leaks?