True or false: In a typical eukaryotic protein-coding gene, the transcription start site is necessarily the first nucleotide of the protein-coding sequence.
5 Transcription and RNA Processing Online Quiz Questions
Use this free practice quiz with 20 questions to review 5 Transcription and RNA Processing, test your knowledge, and prepare for your next test or exam.
What DNA region is where transcription machinery assembles and helps determine where transcription begins?
A DNA template strand has the sequence 3′-TAC GGT-5′. What RNA sequence is synthesized from this template?
- A
5′-AUG CCA-3′
- B
5′-UAC GGU-3′
- C
5′-ATG CCA-3′
- D
5′-AUG GGU-3′
Complete the directions for transcription: RNA polymerase reads the template strand and synthesizes RNA .
A transcribed region contains a 5′ UTR and a 3′ UTR. What is true of these regions in mature mRNA?
- A
They are removed from pre-mRNA along with introns.
- B
They are transcribed and retained in mature mRNA but are not translated.
- C
They mark the DNA positions where transcription must terminate.
- D
They are added to the RNA after it leaves the nucleus.
Which enzyme adds the stretch of adenine nucleotides that forms the poly(A) tail during pre-mRNA processing?
During typical pre-mRNA splicing, the spliceosome removes and joins neighboring exons.
True or false: In eukaryotic protein-coding genes, cleavage of the RNA and termination of transcription are always the same event.
- A
True
- B
False
Which functions are supported by the 5′ cap on eukaryotic pre-mRNA? Select all correct choices.
- A
It helps protect the RNA from breakdown.
- B
It removes introns from the pre-mRNA.
- C
It supports export of the RNA from the nucleus.
- D
It supports recognition by translation machinery.
Which statements describe transcription or mRNA processing in bacteria? Select all correct choices.
- A
Transcription takes place in the cytoplasm.
- B
Ribosomes can translate an RNA while it is being transcribed.
- C
Bacterial cells use a nucleus to separate transcription from translation.
- D
Bacterial mRNAs typically undergo less processing than eukaryotic pre-mRNAs.
A eukaryotic gene undergoes alternative splicing. What outcome can this produce?
- A
It causes RNA polymerase to use both DNA strands as templates.
- B
It adds the poly(A) tail before transcription begins.
- C
It joins different combinations of exons, allowing one gene to produce multiple mature RNA forms.
- D
It converts untranslated regions into protein-coding sequences.
True or false: For many eukaryotic protein-coding genes, pre-mRNA processing can begin while transcription is still in progress.
- A
True
- B
False
Name one RNA class that functions as an RNA molecule rather than being translated into protein.
Describe the three broad stages of transcription in order, including the main event that occurs during each stage.
Which description best explains the role of the 5′ cap added to eukaryotic pre-mRNA?
- A
It removes introns and joins neighboring exons.
- B
It helps protect the RNA and supports export and translation machinery recognition.
- C
It directs RNA polymerase to the DNA template strand.
- D
It adds a long stretch of adenine by copying the DNA template.
Which statement correctly distinguishes a promoter from a transcription start site (TSS)?
- A
The promoter is the first DNA position copied into RNA, while the TSS is where translation begins.
- B
The promoter is always part of the mature mRNA, while the TSS is removed during splicing.
- C
The promoter is a region where transcription machinery assembles, while the TSS is the first DNA position copied into RNA.
- D
The promoter is the start codon, while the TSS is the stop codon.
During transcription, what is the name of the DNA strand that RNA polymerase reads 3′ to 5′?
How is the poly(A) tail of eukaryotic mRNA produced?
- A
A stretch of adenine is added to the RNA during processing.
- B
The tail is transcribed as a long run of adenines directly from the DNA template.
- C
The tail is created when introns are joined together.
- D
The tail is added to the 5′ end by the spliceosome.
Which statement describes transcription and translation in bacteria?
- A
Transcription occurs in the nucleus, and translation begins only after RNA export.
- B
Transcription and translation are separated because bacterial mRNA must undergo extensive splicing.
- C
Transcription occurs at the cell membrane, and translation occurs in the nucleus.
- D
Transcription occurs in the cytoplasm and can take place while ribosomes translate the RNA.
Which statement best describes the relationship between RNA cleavage and transcription termination in eukaryotic protein-coding genes?
- A
Cleavage and termination are always the same event and occur at the TSS.
- B
RNA cleavage and transcription termination are linked but are not always the same event.
- C
Cleavage removes introns, whereas termination adds the 5′ cap.
- D
Termination occurs only after mature mRNA has been exported to the cytoplasm.