01
True or false
1 point
True or false: A gel showing a DNA fragment's position reveals the fragment's exact base sequence.
Free Practice Quiz Question List
03 DNA Technologies and Genetic Engineering Online Quiz Questions
Use this free practice quiz with 20 questions to review 03 DNA Technologies and Genetic Engineering, test your knowledge, and prepare for your next test or exam.
20 questions
02
Choose one
1 point
In a PCR cycle, which step uses heating to separate the two DNA strands?
- A
Primer annealing
- B
Denaturation
- C
Extension
- D
DNA ligation
03
Fill in the blank
1 point
Restriction enzymes cut DNA at .
04
Written response
1 point
Which PCR method monitors fluorescence during amplification to estimate the starting amount of target DNA?
05
Choose one
1 point
A DNA sample is separated by gel electrophoresis. Which statement best describes how fragment size affects migration?
- A
Longer fragments generally travel farther because they contain more DNA.
- B
Fragments move toward the negative electrode, with shorter ones traveling farther.
- C
Shorter fragments generally travel farther through the gel toward the positive electrode.
- D
All fragments travel the same distance, regardless of size.
06
True or false
1 point
True or false: When RNA is the starting material for RT-PCR, reverse transcriptase can first convert it to complementary DNA.
- A
True
- B
False
07
Written response
1 point
Which sequencing method is useful for reading a selected DNA fragment, such as a PCR product or plasmid insert?
08
Choose all
1 point
Which tasks can bioinformatics software perform when interpreting sequencing reads? Select all that apply.
- A
Compare reads with a reference sequence.
- B
Separate DNA fragments by size in a porous gel.
- C
Identify substitutions and insertions or deletions.
- D
Estimate how common a variant is in a sample.
09
Choose one
1 point
In recombinant DNA cloning, which enzyme joins a DNA fragment to its carrier molecule, often a plasmid?
- A
Reverse transcriptase
- B
DNA ligase
- C
Cas9
- D
DNA polymerase used in PCR
10
Fill in the blank
1 point
In CRISPR–Cas9 editing, repair by can create small insertions or deletions that disrupt a gene.
11
Choose all
1 point
After a genome-editing experiment, which checks can help evaluate the outcome? Select all that apply.
- A
Test whether the intended target changed.
- B
Assume every cell was edited if any edited cell is detected.
- C
Examine likely off-target sites and unexpected changes.
- D
Estimate the proportion of cells that were edited.
12
Choose one
1 point
A lab needs to analyze many DNA fragments in parallel for a targeted gene panel. Which approach is best suited to this task?
- A
Gel electrophoresis
- B
Sanger sequencing
- C
Next-generation sequencing
- D
Restriction enzyme digestion
13
Open ended
1 point
Sequencing identifies a DNA difference in a person with a particular disease. What can the sequence result establish, and why is it not sufficient by itself to conclude that the difference caused the disease?
14
Choose one
1 point
Two DNA fragments are run through the same gel, and one is shorter than the other. What should you generally expect, and what can the gel establish about their sequences?
- A
The shorter fragment travels a shorter distance, and the gel reveals its exact base sequence.
- B
The shorter fragment generally travels farther, but the gel does not reveal its exact base sequence.
- C
The longer fragment generally travels farther, and the gel reveals its exact base sequence.
- D
Both fragments travel the same distance unless their sequences are known.
15
Choose one
1 point
During PCR, the DNA strands have just been separated by heating. Which step allows primers to bind to sequences on either side of the target?
- A
Denaturation, when primers are extended to copy the target.
- B
Extension, when the two DNA strands are separated by heating.
- C
Primer annealing, when cooling allows primers to bind to complementary sequences.
- D
Gel electrophoresis, when primers bind to the target DNA.
16
Choose one
1 point
A researcher wants to estimate how much target DNA was present before amplification. Which approach fits this goal?
- A
Monitor fluorescence during amplification to estimate the starting amount of target DNA.
- B
Measure how far DNA fragments travel through a gel to determine their starting amount.
- C
Read every base in the sample before amplification begins.
- D
Use fluorescence only after amplification to join DNA fragments together.
17
Choose one
1 point
A project needs to analyze a large collection of DNA fragments in parallel. Which method is the best fit?
- A
Sanger sequencing, because it reads many DNA fragments in parallel.
- B
Next-generation sequencing, because it reads many DNA fragments in parallel.
- C
Gel electrophoresis, because it determines the exact base sequence of many fragments.
- D
Restriction digestion, because it reads whole genomes without sequencing.
18
Written response
1 point
In CRISPR–Cas9 editing, what is the name of the required DNA signal near the complementary target sequence?
19
Written response
1 point
In recombinant DNA cloning, which enzyme joins a DNA fragment to a plasmid vector?
20
True or false
1 point
True or false: Identifying a sequence difference in a sample is, by itself, proof that the difference causes a particular disease.
- A
True
- B
False