Use this free practice quiz with 20 questions to review 05 Biotechnology Laboratory Methods, test your knowledge, and prepare for your next test or exam.
A laboratory checks a pipette between scheduled calibrations to confirm that it remains fit for use. This check is distinct from calibration, which compares instrument readings with known references and may establish corrections.
True
False
A positive control checks whether key parts of an assay can work, but it does not prove that every sample in the run is free from error. Is this statement true or false?
True
False
Which analytical method separates mixture components as they move through a stationary phase with a mobile phase?
What term describes the range of values reasonably associated with a measurement result?
A spectrophotometric sample is visibly turbid, and its absorbance is being used to estimate concentration. Which approach best supports a trustworthy interpretation?
Assume the absorbance directly reflects the target concentration because spectrophotometry is unaffected by sample appearance.
Assess possible effects of turbidity or interfering substances, and use a suitable blank and calibration range.
Discard the calibration series and report the instrument's unadjusted reading.
Treat the reading as definitive if it is within the instrument's display range.
A gel shows a band at the expected size for a target nucleic acid. What conclusion is justified by that observation alone?
The band proves the sample is pure because it has the expected size.
The marker confirms that the sample was collected and stored correctly.
The band alone does not establish identity or purity, even if its size matches the expectation.
The band proves that the separation method was validated for every intended use.
Between scheduled calibrations, a laboratory measures a pipette's performance against an internal acceptance criterion to confirm it remains fit for use. How should this activity be classified?
The pipette is undergoing a routine performance check, not calibration.
The pipette is being calibrated because any check of equipment counts as calibration.
The pipette's traceability is established automatically by the check, without records.
The pipette is being validated for every possible laboratory use.
An assay produces plausible values and its controls pass, but the sample was mislabeled before testing. Which interpretation is best?
The analytical phase is valid because the instrument completed the procedure.
The post-analytical phase can resolve the issue by reporting the instrument output.
The result may be unreliable because a pre-analytical sample-identification error can invalidate later testing.
The result is valid if the assay controls passed, regardless of the sample label.
A laboratory is choosing an analytical method for a new task. Select all factors that should guide the choice.
What must be identified or measured.
The sample matrix.
The required sensitivity.
The intended use of the result.
Whether the method eliminates the need for controls.
The file format used to store the final report.
Which practices support the integrity and reviewability of laboratory records? Select all that apply.
Preserve the original entry when making a correction.
Make a correction traceable to its author and date, and explain it when needed.
Silently overwrite an incorrect entry so the record is easier to read.
Retain raw data as well as processed results.
Reconstruct missing observations from memory without documenting that they are reconstructed.
Record relevant sample, operator, method, equipment, reagent, observation, and review details.
Explain the difference between technical replicates and biological replicates, including what kind of variation each helps assess and why many technical repeats do not replace independent biological samples.
Before testing a cell-culture sample, a laboratory worker checks its identity and storage history. Which workflow phase includes these checks?
Analytical
Pre-analytical
Post-analytical
Calibration
True or false: In gel electrophoresis, a band at the expected size by itself establishes the identity and purity of the sample.
True
False
A laboratory needs to estimate the starting amount of a selected DNA target by tracking signal as amplification proceeds. Which method best fits this purpose?
Gel electrophoresis, because it directly estimates the starting amount from band size
Spectrophotometry, because it tracks the target during DNA amplification
Quantitative PCR, because it tracks signal during amplification to estimate starting target amount
DNA sequencing, because it measures amplification signal in real time
A laboratory checks a pipette between scheduled calibrations to confirm that it remains fit for use. How should this activity be described?
A routine performance check to confirm the pipette remains fit for use
A new calibration, because every check compares the instrument with known references
A validation study, because the pipette is being used in a laboratory
A measurement of biological variation
A positive control produces its expected result in a run. What conclusion is justified?
It proves that every sample in the run is free from error.
It checks that key parts of the procedure can produce an expected result.
It replaces the need for a separate control that checks the measurement process.
It is expected to contain no target analyte and reveal background signal.
A laboratory adopts an established assay and checks that it performs as expected in its own setting. What is this process called?
An electronic laboratory system must preserve a traceable record of changes to data. What system feature provides this record?
When correcting a laboratory record, preserve the and make the correction traceable to its author and date.
If a required control fails, the run should not be treated as merely because the sample results look plausible.