01
True or false
1 point
True or false: Following aseptic technique guarantees that a workspace is completely sterile.
Free Practice Quiz Question List
04 Cell Culture and Aseptic Technique Online Quiz Questions
Use this free practice quiz with 20 questions to review 04 Cell Culture and Aseptic Technique, test your knowledge, and prepare for your next test or exam.
20 questions
02
Choose one
1 point
Which statement correctly distinguishes defined and complex microbial media?
- A
A defined medium has known chemical ingredients.
- B
A complex medium has known chemical ingredients.
- C
A selective medium contains only extracts and digests.
- D
A differential medium supports all organisms equally.
03
Written response
1 point
What is the classification of a medium whose chemical ingredients are known?
04
Fill in the blank
1 point
A microbial medium containing ingredients such as extracts or digests whose exact composition is not fully specified is called a medium.
05
Choose one
1 point
A researcher wants to distinguish organisms by a visible color change linked to a metabolic reaction. Which medium property is most relevant?
- A
It supplies extra nutrients for organisms with demanding requirements.
- B
It contains only ingredients with fully specified chemical compositions.
- C
It reveals visible differences in growth or substance use, sometimes with a color indicator.
- D
It prevents all organisms from growing so cultures remain uncontaminated.
06
Choose all
1 point
Select all practices that help reduce contamination or culture mix-ups during routine handling.
- A
Prepare a clean, organized work area.
- B
Leave culture vessels open throughout the work session.
- C
Open culture vessels only when needed.
- D
Keep containers closed when they are not in use.
- E
Work with several cultures at once to save time.
- F
Handle one culture at a time to reduce mix-ups and cross-contamination.
07
True or false
1 point
True or false: Adding antibiotics is an adequate substitute for aseptic technique because it prevents contamination from entering a culture.
- A
True
- B
False
08
Written response
1 point
What term describes animal cells growing attached to a surface as a layer?
09
Fill in the blank
1 point
In plant tissue culture, cells, tissues, or organs taken from a donor plant are often called .
10
Choose one
1 point
Which set best describes the core components of a balanced basal medium for animal cells?
- A
Agar, plant growth regulators, and a sugar source only.
- B
Salts, amino acids, vitamins, and an energy source.
- C
Antibiotics and a color indicator, without other nutrients.
- D
A carbon-dioxide atmosphere as the sole source of nutrients.
11
Choose all
1 point
Select all conditions that may need to be matched to a particular animal cell line.
- A
The choice of medium.
- B
Temperature.
- C
Gas conditions.
- D
The color of the culture vessel label.
- E
Whether the cells need an attachment surface.
12
True or false
1 point
True or false: If a culture looks normal during visual inspection, contamination has been ruled out.
- A
True
- B
False
13
Choose one
1 point
As a closed batch culture grows without transfer to fresh medium, which change is expected over time?
- A
Nutrient levels rise while waste products disappear.
- B
The culture remains unchanged because closed systems prevent growth.
- C
Waste accumulates, but nutrients are not used.
- D
Nutrients are gradually used and waste products accumulate.
14
Open ended
1 point
Explain why one culture-medium recipe should not automatically be used for microbial, plant-tissue, and animal-cell cultures. Give one relevant medium feature for each culture type.
15
Choose one
1 point
What is the role of buffering in animal-cell culture medium?
- A
It supplies a gelling surface for all animal cells.
- B
It helps keep pH within a suitable range.
- C
It guarantees that every cell line uses the same medium.
- D
It eliminates the need for an energy source.
16
Choose one
1 point
What is the purpose of a differential microbial medium?
- A
It provides extra nutrients for organisms with demanding requirements.
- B
It has known chemical ingredients in every case.
- C
It makes visible differences in how organisms grow or use particular substances.
- D
It inhibits some organisms while favoring others.
17
Written response
1 point
What is the term for a microbial medium whose chemical ingredients are known?
18
Choose one
1 point
A plant tissue culture produces roots when the aim is to promote shoots. Which medium adjustment could affect whether the tissue forms roots or shoots?
- A
Change the sugar source to determine the species’ exact nutrient needs.
- B
Change the balance of auxins and cytokinins.
- C
Remove all mineral nutrients from the medium.
- D
Use the same formulation regardless of tissue and growth stage.
19
Choose one
1 point
An animal-cell culture grows as a monolayer attached to its vessel. What is the appropriate general approach when passaging this culture?
- A
Transfer the entire vessel without changing the culture conditions.
- B
Add a gelling agent to the culture.
- C
Move the cells directly into a plant medium.
- D
Detach the cells and transfer them to fresh vessels.
20
Written response
1 point
In a closed batch culture, which growth phase commonly follows exponential growth as nutrients are used and waste products accumulate?